FastQCFastQC Report
Mon 10 Oct 2016
362711M_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename362711M_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762492
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAG409.4935397E-486.7505953
TGTAAGA500.002301808469.4004752
TCTATAC1552.2406966E-644.77453
ATACCAT1355.200094E-542.8397986
GTGTAAG1901.858316E-742.6143261
TCTAGGA1406.44424E-541.3098032
AAGGGTA1755.1614625E-639.6574135
TAGGACT2352.2133463E-839.3761564
CATATAC2308.309944E-735.2031361
GTACTAG1651.6954448E-435.0507431
TACATAC1350.002681633534.271842
ACCCTAT1400.003204876333.0478441
CATTACT1450.00380585131.908264
TACATTG1500.004492715530.8446565
ATAGGAC3401.6603735E-830.6178573
TTTACCA1550.00527414629.8496673
GTATAGG1550.00527414629.8496671
CTAGGAC1954.518181E-429.6583213
CATAGGA3602.8949216E-828.9168642
CCTTTAT1600.00615934828.9168641