FastQCFastQC Report
Mon 10 Oct 2016
361703M_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename361703M_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107842
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAA300.007333695143.37115235
TTAGGAT450.00151736277.089353
TATAGGG1551.1761029E-437.3012962
TTATAGA1250.00184120237.0028883
TCCTAGG2450.00238112535.111305235
ACAATAC1802.831818E-432.120563
CCCTAAG1450.003811980631.899041
ATATAGA1450.003811980631.899041
CATTATG1600.00616924228.9085061
GTATAGG2803.8691305E-628.9085041
GTGTAGG9100.027.9554771
TAGATAG2905.0839935E-627.911665
CACAATA2250.001044933125.6964472
TATTGGA4101.0257099E-725.3830762
TATTGAA2951.8280618E-423.5187842
TAGTACT2650.00270797621.817744
AGTAAGG2700.003018211321.4137062
GTATTGA6151.0702934E-820.6825081
CCTTATG2800.003726582520.6489321
TATTACG2850.00412885520.286672