FastQCFastQC Report
Mon 10 Oct 2016
361703M_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename361703M_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107842
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTT150.0059265867153.965015
GTATATA451.1873939E-5102.643341
GTTTTAA600.004772315357.7368771
TCTTAAG650.00655052653.2955782
TCTAAAA903.678443E-451.321672
TATAGCA954.8029248E-448.6205252
TCTATAC1707.858034E-847.5480163
TAGCATG1150.001230619340.1647835
GTACATA1951.0973905E-535.5303881
TATAAGG1300.002247310535.5303843
TAAGGAG3004.98585E-934.6421245
GTATAGA1350.002704286734.2144471
ATCTATA1400.003231929132.99252
GTATAGG2451.3804911E-632.99251
CTTAGAG1400.003231929132.99253
TCATATA1450.003837952431.854832
GTCCTAG2202.5053814E-531.4928421
ATAAGGA3001.9244544E-730.7930014
GTATTGT1600.006211181628.8684391
ATAGCAC2404.5376662E-528.8684393