FastQCFastQC Report
Mon 10 Oct 2016
358758H_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename358758H_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182984
Sequences flagged as poor quality0
Sequence length32-241
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAA1006.3544995E-445.9367071
CTAGGAT1752.499241E-432.811933
GTCCTAG3401.782064E-830.399291
ATCTATA1904.0481304E-430.2215161
TAATAGA1550.005463325429.6365832
ATATAGT2404.7109446E-528.710441
TCTATAG2056.3155364E-428.0101852
CTATATC3406.0379534E-727.0215914
CCTTATA2608.125442E-526.5019452
GTATAGG3109.004652E-625.932011
TCCTAGG3558.801271E-725.8798352
GGATATA3201.15192215E-525.1216341
TATAGGT3701.2624587E-624.8306524
TGTAGTA5251.8644641E-924.0620822
GTGTTAA4002.487519E-622.9683531
CTACCAT3052.4004804E-422.5918224
CCTAGGT3102.679684E-422.2274383
TCTAAGC2600.002523300722.0849553
TCTAACG3152.9859183E-421.874622
CTATATA2700.0031411221.2669934