FastQCFastQC Report
Mon 10 Oct 2016
358758H_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename358758H_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182984
Sequences flagged as poor quality0
Sequence length32-241
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACA1901.9914296E-742.2439842
TATTAGG1200.001570502438.220752
CTATAAG2453.453897E-837.4407272
TAATACT1551.2360401E-436.987824
CCTTATA2206.2869367E-736.4834372
TTAGGAG1951.1517901E-535.2806853
TATAAGG1951.1517901E-535.2806853
TTATAGG2708.1205144E-833.9739954
GTATAGA2202.6292259E-531.2715171
TATACCA1904.085403E-430.1742745
GTCCTAA2703.1130076E-629.7272471
CTTATAG2703.1130076E-629.7272473
TATAGAT3102.728757E-729.5902563
CCTATAA3102.728757E-729.5902561
AGCTATA1600.0064290528.6655582
CTATAGA2506.2883744E-527.5189362
CCTATAC2107.337672E-427.300533
TGTTAAT1700.00864264526.979352
CTATTAC4458.636562E-925.7667941
GTATAGG3201.1659795E-525.0823631