FastQCFastQC Report
Mon 10 Oct 2016
355829P_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename355829P_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971108
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT657.339017E-571.099581
TAAGACT1101.5609377E-552.516734
CTTATAT700.00876114249.515783
ACTAGAC1304.1904404E-544.4372373
TAGATAG1057.8435586E-444.0140235
CTAAACG1250.001848708136.9717834
TATGCAC2554.5929482E-836.2468453
GTATATG1601.4245528E-436.1052551
AATAGGA1300.002241162635.549792
CTTCATA2350.002338019735.241737235
ATTACAT1350.002696894334.233133
TACAGTG1400.003223101133.010527
GTGCATA3052.2133827E-730.3047371
TTGGACT5053.6379788E-1127.4542944
ACTTTAA350.00824298927.244173190-194
CAATAGA1700.00832748427.1851331
AATAGAT1750.009591781526.4084152
ATACAGT1750.009591781526.4084156
TTCATAG2350.001352954524.58232
TATTGGG3301.3950243E-524.5078092