FastQCFastQC Report
Mon 10 Oct 2016
355419N_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename355419N_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111277
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAT751.5100795E-461.4557081
TATATAC650.00660563253.1828232
TTATACG903.717098E-451.213094
ATAAAAA1300.0086082949.734417235
GACTAAA700.00885418749.3840522
CATATAG2001.3243067E-534.5688322
TCCTAGA2001.3243067E-534.5688322
TTTATGA1350.002732517234.142062
CTACACT1500.004577772730.7278544
TGTAATA1500.004577772730.7278542
GCATATA2303.443571E-530.0598561
TAGAACC5003.45608E-1127.655074
TATATGG2107.1288156E-427.4355835
TCTAGGA1700.00843690827.112812
TTACGTA1700.00843690827.112814
CCTAGAG2158.17943E-426.7975462
GTCCTAT4755.620677E-1026.6847131
TTCAATA2607.940847E-526.5914122
ACTTAGC2250.001066519525.6065442
GTGTTAC3651.0887743E-625.2557721