FastQCFastQC Report
Mon 10 Oct 2016
355419N_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename355419N_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111277
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAT450.00154516276.737524
ATATAAC1253.3984878E-546.042511
CCTTAGG1100.00100455441.8568272
TAAAACT1200.001540919938.368764
GTGTATA1250.001883025636.8340071
GTATACA1909.387009E-636.349351
TATAGAC1300.002282734735.4173132
CTATGCT1350.00274688234.1055644
ACGTAAG1853.415893E-431.1098041
CCCTTAG1550.0054020929.7048471
TGTATAC1954.650056E-429.514432
GTATAGA1600.00630865428.776571
TCTATAC1600.00630865428.776573
ATATAGA2107.173515E-427.4062581
CCTTACA1700.00848103227.083832
TACCGTA2158.23068E-426.7689047
CTAGTAC1750.0097685126.3100056
TAGTACC3558.6256114E-725.9394444
ATAGAAC4509.335054E-925.5791743
GTCCTAT3201.131476E-525.1794971