FastQCFastQC Report
Mon 10 Oct 2016
347487A_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename347487A_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198019
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAT409.5594255E-486.6027453
TATAAAT751.4946106E-461.584172
TGCTAAG956.572627E-660.7738572
GTCCTAA1502.9327566E-853.886151
AATAGTA700.00878159149.487282
GACTAAA1150.001230839440.163592
GTATAAC1300.002247710535.529331
ATAGGAC3353.8562575E-1034.4687543
AGTTAGG1350.002704768434.213432
TAAATGT1400.003232503532.9915244
TTAGGAC1500.004531385430.7920863
GTACCAT1550.00531950829.7987946
TCCTAGA1550.00531950829.7987942
TATTACA1600.006212281528.8675822
CCCTAAA1700.00835166427.1694891
CCTAGAG2158.0806395E-426.8535653
TGCATAG2158.0806395E-426.8535652
TCCTAAC1750.00961961226.3932172
CCTATAC2751.14635965E-425.1935253
GAATAGT3851.7015464E-623.9938341