FastQCFastQC Report
Mon 10 Oct 2016
347487A_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename347487A_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198019
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAG355.636395E-498.915271
TCTAAGT409.5822103E-486.550862
TAGAATG600.00478430757.7005735
TAGACTC1109.920179E-441.964055
CATATAA1150.001234471740.1395261
TTCTATA1250.00185957836.9283642
GTTATAG1300.002254329635.5080451
GTATAGA1951.1022057E-535.508041
TATAGAC2001.3109564E-534.6203422
CCTCTAA1350.002712726634.1929321
TATATGT1853.3651947E-431.1894974
ATATATG1853.3651947E-431.1894972
GTGTTAA1500.0045446930.7736421
CATTACA1700.00837611527.153214
TAGACAG2801.300846E-424.7288175
TAGGACT2951.8533313E-423.4714184
TATACCG5002.78269E-823.0802275
TCGTATA2550.002191413422.6276742
TAAGCCT2550.002191413422.6276744
CCCTACA4603.178684E-722.5784842