FastQCFastQC Report
Mon 10 Oct 2016
346371B_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename346371B_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152770
Sequences flagged as poor quality0
Sequence length32-241
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4956.5683525E-720.9383621
GTGTAAG6801.6098056E-920.3225291
CCGTGCG16500.018.8445269
ATAGGAC5802.9968287E-617.8698083
GTATTAG16150.017.1137071
GTATAAG8852.2937456E-916.9163041
ACCGTGC18700.016.6275238
AGGACTG6256.106944E-616.5831815
GTACTAT9805.002221E-1016.451571
CTAGAGC5154.3676316E-415.6529493
GTCCATA10401.1623342E-915.5024411
GTACTGG14250.015.3547991
GTCCAGA12805.456968E-1215.2948211
TAAGCCC5305.432434E-415.2099424
CTGTGTG6109.199173E-515.1030819
CCAGGGG12002.4368037E-615.077242235
TATACAG10751.8553692E-914.9977115
GTATTAT25700.014.7872091
TAGGACC6251.1314542E-414.7406064
AGGACAG8605.1589814E-714.7298945