FastQCFastQC Report
Mon 10 Oct 2016
346371B_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename346371B_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152770
Sequences flagged as poor quality0
Sequence length32-241
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT5352.2573658E-923.6684174
TCCTAGG3002.1120664E-423.0229152
TTAGGGC3052.3621842E-422.645494
GTCTATA8051.8189894E-1221.449921
CTAGGAC4751.0792797E-519.3877183
CCTAGGC3607.2319567E-419.1857623
ATATAGG10450.018.7267741
ATAGGAC5652.3423927E-618.3368363
TACTCTG4451.4366617E-418.107917
GTCCTAG3200.00827426717.9866521
TAGACTG3850.001135405617.9399345
ATAGGGC4501.5649691E-417.9067123
GTCCTAC7105.856964E-817.8346541
TAGGACC6504.5453817E-717.7099344
CTCTTAG3300.00987863717.44161
GTATAAG10655.456968E-1217.2942091
GTATATG14050.017.2057381
TAAGAGT6706.243954E-717.181284
CACACAG6054.4985245E-617.1244835
TGTAAGA8852.3046596E-916.9094872