FastQCFastQC Report
Mon 10 Oct 2016
344204H_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename344204H_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308275
Sequences flagged as poor quality0
Sequence length32-241
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTC9300.023.462268
GTATATA25450.022.1109541
CTAGTCC10300.021.184379
TATACTG16850.018.4019175
ACACTAG10700.018.245866
GTATTAT39400.018.0715181
CTAGGGG3200.00838884617.9439983
CTAACAC15450.017.839473
GTCCTAT9051.6734703E-1017.7655471
CCCTTAG3900.001258033117.6679361
GTCCTGG14450.017.4845311
CTATACC14000.017.2262384
TATAGAG19450.017.122915
CACTAGT12350.016.7380457
TAACACT26800.016.7120234
GTCTAAG4852.824759E-416.5750731
TCTATAC14600.016.518313
AGTACTG9805.2023097E-1016.4059415
GGACTAC5705.2790074E-516.1181186
TATATAC29500.015.9610333