FastQCFastQC Report
Mon 10 Oct 2016
344204H_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename344204H_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308275
Sequences flagged as poor quality0
Sequence length32-241
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTC8050.028.5225938
CTAGTCC11850.023.251339
GTATATA25850.021.7615811
CACTAGT10950.019.9202337
ACACTAG11650.018.7233076
TAACACT25500.018.4585914
TAGCTAG8401.195076E-917.7671995
CCTATAC9752.5465852E-1117.6620673
TATATCG21050.017.4523035
TAGAGCG8001.2565579E-817.2205165
AACACTA22750.017.1574365
ATAAGAG14200.016.9779743
ACATAGG6807.502349E-716.8828583
TAAGAGG9805.2204996E-1016.4004924
GTTATAC15400.016.4004923
AGACCGT9855.620677E-1016.317246
TAGAGAG5755.704659E-515.9726535
GTATAGG10801.2187229E-1015.94492151
AGAGCGC8052.527304E-715.6874276
AAGACAG9556.557457E-915.6276935