FastQCFastQC Report
Mon 10 Oct 2016
343671Q_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename343671Q_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158666
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAT1057.9298235E-443.9170842
TTAGCAC1355.30514E-542.6971663
TTTGTAA1200.001529418438.427451
GTCTTAT2402.7463102E-838.427451
TCTAGGA1601.4433192E-436.0257342
GTACTAG1601.4433192E-436.0257341
GTACGAT1350.00272642334.1577341
TCTATAG1400.003258366132.9378133
ATTATCC2202.5340456E-531.4406413
GTGTAAG1903.958274E-430.337461
GCCTAAT1550.00536197229.7502821
TATAGGA1600.006261838628.8205852
TAGGATC1650.007277088227.9472354
TATTGTT2158.157744E-426.8098492
ACAGTAC2607.916376E-526.6036198
CTAACAC1750.00969617726.350253
TCTATAC1750.00969617726.350253
CATTAGC2250.001063695425.61832
ATCTATA2450.001746973123.527012
TAAGGCC5053.1259333E-822.8281884