FastQCFastQC Report
Mon 10 Oct 2016
343671Q_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename343671Q_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158666
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAT1755.31574E-639.4902953
CTAGATA1300.002275612435.4400064
TGTAATA1300.002275612435.4400062
GTGTATA1651.7386119E-434.9030381
TATAGAC2252.9725134E-530.7146742
TCTAAGC1500.004587470630.7146722
CTCATAA1500.004587470630.7146721
CCTAGAT2652.5980735E-630.4249133
GTATAGA2005.372822E-428.7950061
TCTTATG1650.007308696427.922432
CCTTACA1700.00845472727.1011832
GGGTTAA1700.00845472727.1011831
GTACTGT3159.964693E-625.5955621
GTATAGG2300.001215427225.0391371
TTACACC3851.739143E-623.933514
TAGTACC3452.0161066E-523.369864
ATACTGT2500.001974987323.0360056
CTTATTC2500.001974987323.0360053
CTATATC4153.3379347E-622.2033774
GTAATAC3703.461209E-521.7908153