FastQCFastQC Report
Mon 10 Oct 2016
341287P_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename341287P_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091017
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGTG1250.00187084736.882665
GTCTAAT1350.00272914234.1506081
GTATACA1802.886376E-432.0161971
TTCTATA1500.00457213230.7355482
TCTAGGG2850.005239668329.888632235
TACTGTT2353.981038E-529.4276522
GTATTGT1600.006268060328.8145771
GCTATAA1600.006268060328.8145772
GCCCTAA2803.9683364E-628.8145771
TATATGT1650.00728431527.9414082
ATATAGG1700.00842654327.1196023
CTATACG2158.167377E-426.8042584
GGCTATA2607.9271995E-526.598071
GTATAGG2209.340695E-426.1950681
ATATAGC2300.001210577225.0561563
GGATATA2801.3117235E-424.698211
CTAGACA2350.001372221724.5230454
GTGTAAT2350.001372221724.5230451
GTCTAGA2851.4791355E-424.2649061
CCATAAA2550.002207116722.5996691