FastQCFastQC Report
Mon 10 Oct 2016
341287P_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename341287P_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091017
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA550.003414528762.830081
TCTAGTA751.5127254E-461.4338572
CCTAATA852.8080988E-454.2063452
GTACTAT650.006614897453.1639141
CTAATGT650.006614897453.1639144
TATAAGG700.00886659549.366493
GTTCTAA1304.2661584E-544.303261
TTAGAGA1300.002274735435.4426084
CCCTAAT1500.00458570730.7169281
TTTTAGA1500.00458570730.7169282
TGTAGAA5350.030.142782
TAAGGGT2056.204869E-428.094754
GTGTAGA7150.027.387471
TAGGACC2955.983542E-627.332864
TTTAGAG1750.00973450526.3287943
ATTACAC2659.060965E-526.0804083
GTATACA2250.001068721625.5974391
TATACCG4509.262294E-925.5974395
GTGTAAC2751.1654534E-425.1320321
GTATTGA6900.025.0409761