FastQCFastQC Report
Mon 10 Oct 2016
340864F_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename340864F_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723710
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAT2605.998845E-835.1663743
TTAGCTA1450.00403699931.5284758
CTCTTAA2253.1349686E-530.4775261
GTATTGT2303.642444E-529.8149721
CTCTATA2455.6038803E-527.9895652
TATATGC2506.430567E-527.4297733
ATCTAAG1700.00878161226.8919321
GTATAGA3109.350344E-625.8075831
TATAGAT3301.5181444E-524.2434862
CTATATG2951.9793132E-423.2455712
TCTAGCA2951.9793132E-423.2455712
GTATAGG5154.2195097E-822.1923731
TGTATAT2600.002595239821.9789852
TATATCT3253.8107426E-421.0998254
GATATAT3253.8107426E-421.0998251
TATAGGA2800.00398841720.4090582
TATGTAG2800.00398841720.4090582
TCTATAC2800.00398841720.4090583
CCTTACA3405.166693E-420.168952
GTACAAG12100.019.8355791