FastQCFastQC Report
Mon 10 Oct 2016
340864F_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename340864F_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723710
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTATA400.001009022885.434131
TATACTG500.002446218968.3473055
TATTAGG751.5986824E-460.753162
TACTAAA903.934701E-450.627632
TATAAAG903.934701E-450.627633
CTATAAA1006.614983E-445.564872
CTTAATA1058.4118266E-443.3951152
GTACTAT1200.001622170237.9707221
GTGTTAA1250.00198223936.4518971
TATTAGA1250.00198223936.4518972
GTATTAG1300.002402916635.04991
TCTTAGT1300.002402916635.04991
TGTTAGT1651.8558402E-434.518842
CTAGATA2351.1091724E-633.9312864
ATATAGA3251.24382495E-831.5449091
CCCTTAA2253.206795E-530.376581
ATACTGT3401.9297659E-830.1532236
CTCTTAG2005.733427E-428.4780451
CCTATAA2854.9924984E-627.9784281
CCTTAAG2056.624136E-427.7834552