FastQCFastQC Report
Mon 10 Oct 2016
338348D_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename338348D_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241740
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATA550.003427191962.771921
ATTAATA600.0048371157.5409242
TATTAGG650.006639385653.11472
GTGTTAA1150.001251422240.028471
TTAATAC1150.001251422240.028473
TGTAATT2401.2068613E-633.565542
TTATGAG1903.999014E-430.2846973
TTAGGCT1550.00540793929.698544
TAAGGGT2753.4907735E-629.2935624
TTAGGGT2056.2390533E-428.0687454
TATTGGA3754.5256456E-827.6196422
CCCTTAG2506.134111E-527.6196421
GACTTAG2107.1828975E-427.4004381
GTTAACA1700.008490195527.0780833
CTTAGGC3351.6164347E-524.0469533
ATACCAT2400.001565250923.9753866
GTATACA2951.8885138E-423.4064771
ATCTAGA2550.002227027422.565071
AATAGAT2600.002492954522.1311242
TACTGAA3653.137019E-522.070492