FastQCFastQC Report
Mon 10 Oct 2016
338348D_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename338348D_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241740
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATG1150.006413358353.579388235
ATATATA700.0089483649.2523464
ATATAAC1552.3438388E-644.485991
GTCTAAG1704.4238404E-640.5607571
GTGTTAG1752.4890687E-432.8348961
TCTATAC2101.8836481E-532.8348963
GAGTTAG1802.9364933E-431.9228151
TTCTAGG1450.00392916431.702662
TTAGCTT1450.00392916431.702664
GTCTAGT1450.00392916431.702661
GTATAGG2901.4910438E-731.702661
TCCATAA1904.031685E-430.2426662
GTACTAG2005.443979E-428.7305361
TATACAG1600.006358463328.7305362
GTGTAAG2404.6887115E-528.7305341
CTTTTTT3656.15049E-428.135296235
CCTTAAG1650.007389297227.8599112
CTTAGCT1650.007389297227.8599113
TATGTAC2905.3347994E-627.7398283
TTAACGT1700.00854789827.0405034