FastQCFastQC Report
Mon 10 Oct 2016
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702761
Sequences flagged as poor quality0
Sequence length32-241
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC62400.8879263362651029No Hit
CCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC7800.11099079203313786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTAG1552.1635224E-645.0023273
GCCTAAT1951.0472273E-535.7710844
AAGCCTA2953.956302E-935.4679372
TAGTATC1903.768002E-430.5915097
ATTCCTA1550.00514461330.001552
AGCCTAA2055.8768597E-428.3551243
CTATATT2904.8728853E-628.0617964
TAGGACA2556.546822E-527.3543574
AAGACTA2250.001012365525.834672
GTGTACT3301.3288582E-524.6603684
GCCTAGT2400.001474776124.2200014
CATGCAT3552.3384324E-522.9237211
CAAGACT2650.00262421.9350971
ATCGTAA2750.003253100721.1374571
AGAGACT2800.003612727220.7585246
TATACCA3455.0779246E-420.2184354
GTATTAC3000.005383481719.3760011
GTCTATG3657.421583E-419.1105791
CCCATAT3657.421583E-419.1105791
TATGTGC3708.132654E-418.8523273