FastQCFastQC Report
Mon 10 Oct 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702761
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG1057.5718906E-444.328491
GTATAGA2152.0306543E-532.4731981
CTATAGC1450.00369567132.0999454
TATAATG1450.00369567132.0999452
CTTACAC2055.845531E-428.3810463
CTATTAC2106.730121E-427.7053071
ATATACA2955.526348E-627.6113914
TAGACAG1750.00926290426.5970945
GTTCTAT2658.453075E-526.3461781
ACTATGC2300.001144723325.296158
TAGGGGT2350.001297620124.7579354
GTGTATG2500.001860381123.2724591
ACAAGTG2500.001860381123.2724595
GTATGAG2550.002087423322.8161351
GTGTACA3602.5870128E-522.6261
ATATAGA2650.002610116521.9551491
TCATACA3303.7389615E-421.1567784
AGTGTTG3354.1384858E-420.8410075
ATACTGG3404.5735665E-420.5345211
TACACCG8102.910383E-1120.1120015