FastQCFastQC Report
Mon 10 Oct 2016
BS546900_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS546900_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152195
Sequences flagged as poor quality0
Sequence length32-241
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAT903.632398E-451.4530033
TATAGGA2405.475158E-1043.413472
CAAGTCA2550.005191859329.945484235
ACTAGTA1550.005252612829.8759352
TAGGGTG2300.001179798425.167235
GTATAGA5601.3278623E-1024.8076971
GTATATA3351.5434642E-524.190591
TATACAG9200.022.6505075
TATTATA4855.1313145E-721.4829542
TCTAATG2700.002997887321.4387512
GTATAGG4905.663351E-721.263741
GTATAAA9800.021.263741
GTGTAGT5558.11815E-820.8593251
TATAGCA5006.8772533E-720.8384672
TATACAC5608.9246896E-820.673083
GTACATA4506.4409815E-620.5812021
GTATAAG5108.31802E-720.4298671
ACAGTGT4557.0869683E-620.3550348
GTCCAAG2900.00453544219.9602181
ATAGGAG4107.336675E-519.7654843