FastQCFastQC Report
Mon 10 Oct 2016
BS546900_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS546900_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152195
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA3001.8495848E-730.932911
TACTAAC3354.859885E-727.7011152
TAGACTG3159.432015E-625.7774265
TATATAC4351.7636012E-723.9996723
GTACAAG3901.8294977E-623.7945481
TATACTG7550.023.0460415
TAGGCAG3652.9504206E-522.2462715
TAGACAG3152.7902165E-422.0949365
TCTATAC5855.800757E-921.8116683
TGTACTA3203.1037218E-421.7497025
GTATTAG3753.6347643E-521.6530381
GTGTATA4855.034617E-721.525481
CCTTAGG3253.4465553E-421.4150942
TATAGAA6007.767085E-921.2663762
TATAGAG6058.547431E-921.090622
GTATAGA7751.6370905E-1120.9545521
GTATATG6701.2223609E-920.7758351
TACACTC3404.6737082E-420.4703085
GTATAAG5209.833657E-720.0766491
TATACCG12300.019.804615