FastQCFastQC Report
Mon 10 Oct 2016
BS546899_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS546899_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198447
Sequences flagged as poor quality0
Sequence length32-241
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTA600.004719950757.898322
GTATAAG6000.034.738991
GTATATG8050.028.769351
CTCTTAG1700.00823748927.2462671
TATACTA1750.00948819826.4678045
GTCTTAG2209.090568E-426.317421
GTATAGA6300.025.7325861
TCTATAG2350.001335544724.637583
CTACGTA2851.433217E-424.3782394
GTATACA6252.0008883E-1124.0857011
CTATACT3901.8575192E-623.7531554
GTATAGC9100.022.904831
TGTAGGA2600.002404847622.2685852
TACTGAG5754.8512447E-922.15242
ATACTGT8151.8189894E-1221.3122636
GTGTAAG4354.7931517E-621.2959351
ACAGTGT4405.2918913E-621.0539348
GTATATA4956.2302934E-721.0539341
TTACACT5507.360177E-821.0539344
CTTGGGG4650.00381579720.563612235