FastQCFastQC Report
Mon 10 Oct 2016
BS546899_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS546899_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198447
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG5700.032.561541
GTATATA4007.094059E-1131.9001311
TATAGGA2404.3993015E-529.000122
ACTTAGT1600.0060748129.0001181
GTATAGT4355.991751E-926.6667791
TAGGACT1750.009407226.5143974
GTATAGA8250.025.3091961
TAGTAGT2450.00168500323.6735674
TAGACCC4052.5396075E-622.9136734
TAGACAG4102.8251889E-622.634245
GTATAAG6356.2573235E-1021.9213521
GTATGAG7459.094947E-1221.798751
GTGTAAG3753.634423E-521.6534231
AATCCTA2750.003296208621.0909965
TAGATCT3854.4520144E-521.0909964
TCTATAC7701.4551915E-1121.0909963
GTATTAT10100.020.6733531
TTAGACA3404.6732987E-420.4706734
GTATAGC11350.020.4406131
TGCTATA4709.220086E-619.7447642