FastQCFastQC Report
Mon 10 Oct 2016
BS546872_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS546872_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322144
Sequences flagged as poor quality0
Sequence length32-241
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA1202.590838E-548.2069051
TATAGGA2905.0636227E-627.9267622
GTATATG8550.024.3571761
CTATTAG2400.00151749624.1034531
GTATAGG5802.0736479E-1023.9372251
ATAGACT4351.8089668E-723.9372234
GTGTAGG6402.7284841E-1123.5008681
ATAGGAC3502.1770607E-523.1393173
GTATAAG6053.5288394E-1022.9480841
TAAGAGT3052.283146E-422.7599834
GTATTAA11800.022.551031
TATATAC4153.211373E-622.3029563
TACAAGA3753.7090846E-521.5966952
TCTATAC9200.021.3787163
TAGACTG3253.508134E-421.3593675
TATATAG3253.508134E-421.3593675
GTATACG7101.1277734E-1021.183881
GTATAGC9950.020.9300331
TTATATA6651.1514203E-920.8775774
TGTAATA9450.020.8131432