FastQCFastQC Report
Mon 10 Oct 2016
BS546872_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS546872_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322144
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG8400.026.208861
GTGTAGG6850.025.3731581
TAATAGA5151.3460522E-924.7490944
GTGTTAG4251.424014E-724.5373361
TGTAGGA2550.002140413322.7197552
TATAGCA5653.932655E-922.5589082
TAGAGTG2700.002982915821.4575464
TTATACA7601.2732926E-1121.3446122
GTATAAG5456.639311E-821.2606891
GTATACA5507.3121555E-821.067414
GTATAAA12850.020.7395131
TATATAG3955.4716475E-520.5340565
TATAGGG6301.3798854E-820.23142
CTATAAT5209.940377E-720.0545541
GTATAGA8103.092282E-1120.0270441
CTAATTG3505.725227E-419.8635583
ATATAGG8805.456968E-1219.7506961
TAATACA7102.5520421E-919.5837884
TAAGAGA5951.6762351E-719.4740774
TACACTG7152.7848728E-919.4468385