FastQCFastQC Report
Mon 10 Oct 2016
BR679314_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBR679314_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144339
Sequences flagged as poor quality0
Sequence length32-241
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC2354.4201443E-1044.3780141
TACACAG5051.8189894E-1229.8294455
GTATAAG5603.6379788E-1226.8997691
ATCCTAT3108.399054E-626.1655331
TATTAGG2450.001695523723.648152
ATTATAC4952.3945177E-823.4092793
TATTATA5653.930836E-922.5599162
GTGTACG7750.022.4275991
GTATAGG4703.7586324E-722.1890071
CTTATAC5805.318725E-921.9764693
GTATAAA10150.021.6910611
TCTATAC7101.10958354E-1021.216723
ATTACAC7651.2732926E-1121.206053
TATATAG2750.003316711621.0683525
CTATTAG2750.003316711621.0683521
ATATACT7351.7644197E-1020.4950644
TAGTAGT2900.00451131819.978614
TAATAGT4658.484218E-619.9356464
GTATAGA7002.1373125E-919.8644431
ATAGGAG4157.996081E-519.5453383