FastQCFastQC Report
Mon 10 Oct 2016
BR679314_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBR679314_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144339
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA409.360183E-487.0629041
CTAGAAA950.00401759260.300186235
ATTAGGC3157.625204E-933.166823
GTGTATA4006.91216E-1131.9230651
TATTAGG2556.616786E-527.3138522
TTCTAAG2208.959786E-426.3826982
TCTAAGG2208.959786E-426.3826983
GTATATA3251.1950544E-525.002681
GTATACA4701.3549652E-824.6986941
GTATATG7750.023.96571
GTGTAGA6352.3646862E-1123.7652021
GTAGGAT3001.9954347E-423.2167763
GTGTATG6457.530616E-1021.5971
TTTATAC10900.021.2997933
GTGTAAT7251.4370016E-1020.8150391
TAGGATA2850.00403714720.3655914
TAATACA6401.6187187E-819.9519164
CTTATGT3505.6541467E-419.9000934
TGTAGAC5901.5038131E-719.6752322
TAGACGT4751.0039144E-519.5509684