FastQCFastQC Report
Mon 10 Oct 2016
BI120654_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBI120654_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277593
Sequences flagged as poor quality0
Sequence length32-241
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG3506.366463E-1032.891165
TTAGACT2303.4665754E-530.0310574
ATAGACT5902.746674E-1023.4140474
TATACAG10700.022.5934585
TCTTATA7359.094947E-1221.9274392
GTATAGA6301.4870238E-820.1001511
TTATAGT6901.9390427E-920.0207064
CTAAGTT4600.004823710319.747879235
TAAGACT5851.5015576E-719.6784734
TAGAGCT4107.66207E-519.6544724
GTATAAG5951.7950151E-719.3477421
TCTAATA4301.1044498E-418.7403132
GTACCTA3100.006888809618.567596
TAGACTC3100.006888809618.567595
GTATACG6853.8855433E-818.486271
TATATAT9602.0008883E-1117.9873523
CTAATGT5803.007779E-617.86334
TCTACAC8401.1514203E-917.8160463
TACACTG11000.017.7911265
TAATACT11650.017.7866344