FastQCFastQC Report
Mon 10 Oct 2016
BI120654_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBI120654_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277593
Sequences flagged as poor quality0
Sequence length32-241
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG4651.2732926E-1129.7573491
ATAGTAC4654.3473847E-1027.277573
CTTAGGA2158.146895E-426.8162172
TAGACTG4356.386472E-926.5079845
TAGGACT1750.00968532226.3565124
TATAAGA6950.024.8869932
GTATAGA6651.8189894E-1224.2757341
GTATAAG7700.023.9604641
TCTAAAG4452.3267603E-723.3210723
GTATATA5755.0931703E-922.0592541
TAGTACT2800.0037880220.5910244
ATATAAG10700.020.475561
GTGTATA6201.2138116E-820.458181
CCTAATA4006.256983E-520.1792031
TCGGGGG6406.89341E-519.925138235
TCTAATA4709.7089305E-619.627192
TCCTAAT2950.005123619519.5440221
TAGGATT3556.50823E-419.4889684
CTTATAG4209.103592E-519.2182882
TATAGAC5551.9433883E-618.6988752