FastQCFastQC Report
Mon 10 Oct 2016
BB429684_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB429684_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060233
Sequences flagged as poor quality0
Sequence length32-241
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG4800.031.3743441
CTATACT4058.949064E-825.7430524
TATATAG2709.899247E-525.7430525
GTCTTAC4201.2724013E-724.8236561
GTATAGA6101.4551915E-1124.6880071
GTCCTAC2350.001332282724.6476041
GTACTAT3801.4762209E-624.3881531
GTATATG6751.8189894E-1224.026851
TATAGGA2901.6080723E-423.9676692
TCTATAG2450.00169818623.6415772
GTATGAG6053.4742698E-1022.977271
GTATAGT4052.569148E-622.8827131
GTATAAG4102.858038E-622.6036571
TTAGTAT3152.8153055E-422.0654743
TCCTACA2650.002679567321.8573072
TATAGAA4804.614958E-721.72072
GTACTAC5406.034861E-821.4525431
TACTATA2700.002986568521.4525432
GTCTATA6651.1314114E-920.9041331
CATAGGA3354.2670997E-420.7481332