FastQCFastQC Report
Mon 10 Oct 2016
BB429684_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB429684_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060233
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC700.0086045849.742554
TAGGGTG1759.4811185E-846.426385
CTATAGA2501.5530586E-632.4984661
GTCCTAA1802.7704626E-432.2405431
TCTATAC6000.029.0164893
ATTAGAC1650.007040108628.1372013
TTATATA4654.0381565E-1027.4564634
GTATAGG4254.647518E-927.3096391
CTATAAT3609.063333E-725.7924351
TATATAC2751.10686946E-425.3234833
GTATAAG4801.6971171E-824.1804071
TAGCACT2450.001679351323.686934
TATATAG3152.7790506E-422.1078015
TTCTATA5355.3596523E-821.694572
TATTGTA5405.9138983E-821.4936942
GTATATG7059.822543E-1121.4022331
TAGGAGA4354.696807E-621.3454634
TATAAGA5057.382205E-720.6850222
TACACAG4506.2991294E-620.6339475
TATATGT3455.1366125E-420.1853833