FastQCFastQC Report
Mon 10 Oct 2016
403922K_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename403922K_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762842
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAAA1259.631914E-567.301125235
GTACTAA1058.03314E-443.803051
TGTTACA2301.9317667E-839.994092
TCCTAGA2308.713505E-734.9948272
TCTATAT3005.2004907E-934.4949043
TCCTAAA1350.002761786834.069042
ACTAATA1450.003919434331.719452
GTATAAG1550.005431311729.6730331
GTTCTAA1600.006342752428.7457521
CTAGAGT1650.0073710527.8746683
TATAAGG2506.171556E-527.5959222
ATTATAC350.00776289627.581581205-209
GTCTATA3006.916789E-626.829372
GTGTATA2701.0417744E-425.5517791
TACACTG2701.0417744E-425.5517795
TCCTTAA2300.00122787224.9963072
GTATACA4651.3393219E-824.727531
TCCTACA6151.8189894E-1124.3053512
CTAACAC3401.8254992E-523.6729743
CTTAGGC3401.8254992E-523.6729743