FastQCFastQC Report
Mon 10 Oct 2016
403922K_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename403922K_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762842
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA802.0865891E-457.566672
TTATATC1051.2103652E-554.82542
CCTATAG1752.4628028E-432.895243
CCCTATA2152.185427E-532.1302342
TCATAAA1450.00389444631.7609211
GTATAGA3001.976041E-730.7022231
CTTACAC2703.0162337E-629.8493843
TAATACT1550.005396732629.711834
TCTAGAA1550.005396732629.711832
CTACACT1954.6447513E-429.521374
TTTATGT2005.386708E-428.7833353
TATAGAG1600.00630240128.7833352
TATATGA2056.223797E-428.0813032
TTCTTAA1700.00847263727.0901992
GATTAGT1700.00847263727.0901991
TTCTTTA2659.093624E-526.0679262
TCTAACT2350.001381258624.4964562
TATACAG2350.001381258624.4964565
TCCTAGA3301.4342075E-524.4222262
TTATGGA2500.001980055623.0266672