FastQCFastQC Report
Mon 10 Oct 2016
336256D_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename336256D_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373404
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATATA650.00663442953.125034
TTAAGGT1202.6707059E-547.96014
TGTAACA2308.640109E-735.0317232
TCCTAAG1350.002747345734.1049582
TATATTG1400.00328335132.8869255
GGATAAA3001.979879E-730.694461
TTCTAGG1650.007332681727.9040572
TTATTCC2209.4151555E-426.1600534
TATAGGG2250.001073434425.5787182
TAAGGTG2350.001383139224.4902635
TACTACA4251.518456E-724.3750152
GTATAGG2851.492894E-424.2324711
ATATACA2901.6797955E-423.8146694
TAGGACT3502.2652768E-523.0208474
TAGTACC3002.1136392E-423.0208454
TCTAGAT2550.002224631622.5694583
GTATACA2600.002490273222.135431
CCTTGTA7901.8189894E-1221.8552342
GGTATAT3703.479437E-521.7764761
TATCAAA2650.002781455621.717782