FastQCFastQC Report
Mon 10 Oct 2016
336256D_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename336256D_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373404
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTAA355.6729536E-498.7553251
TTATCCT802.079168E-457.6072774
CCATTAA1306.8529334E-753.1759451
TGTTATA1006.2541786E-446.085822
TATAAGT1250.001874506936.8686564
TACTAAG1300.002272414535.450632
GTATAGG2707.786184E-834.137641
TCTAGTA1400.003267979532.918442
CTAGTAC2451.4052657E-632.918443
TAATGGA1752.4523502E-432.918444
TCCTAGA2152.174569E-532.1528972
CATTAAC1853.3975305E-431.1390692
TTAGGCA2353.9921084E-529.416484
CTTACAC2854.569034E-628.2983113
GCTAGTA1650.00729847827.93082
GTTATAT1650.00729847827.93083
TATATTC1700.00844291327.1093065
TTTACAC2556.965199E-527.1093043
TATAGAT1750.00972463526.3347532
TACTAGC1750.00972463526.3347532