FastQCFastQC Report
Mon 10 Oct 2016
335202Y_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename335202Y_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600981
Sequences flagged as poor quality0
Sequence length32-241
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG5802.3101165E-1023.7365285
GTATATA23250.021.7116951
GTCTTAG12650.020.8593731
GCCCTAG3405.035595E-420.2458611
GTCTAGG5152.2305621E-517.8216021
TAGCCTG3900.001266681517.650245
GTATTAC25450.017.1300961
TCCTATA13750.016.6874982
TATACTG15350.016.4428945
CTAAGAC14900.016.1695163
CTCTTAG7902.0574771E-715.9745841
TAGGACT8303.604364E-715.2047244
GTATTAT43050.015.1902751
GTCCTAT9106.1923856E-815.1287761
GACTGTG7602.4104884E-615.0956017
TTACCCC6109.5040705E-515.0461074
CCTATAC11553.3833203E-1014.8995523
CCACGTA16950.014.8907629
GTACTAC19300.014.8609531
TAAGACT15550.014.7558274