FastQCFastQC Report
Mon 10 Oct 2016
335202Y_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename335202Y_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600981
Sequences flagged as poor quality0
Sequence length32-241
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG3556.65015E-419.427051
CTAGGGC3000.005751719719.1572323
GTCTTAG13250.019.084941
CTTATAC16200.017.7381763
GTAGGAC10403.6379788E-1217.6835983
TAGGCTG3950.001362236817.4597555
TTATACT28250.016.6820494
CTTAGAC8301.9508661E-816.618323
ACTAGGC4200.002053980716.4204838
GTATATG19600.016.4204831
GTCTAGA7051.0806198E-616.3040261
TACACGG7952.1634878E-715.9041175
TATATAC25350.015.8698953
TACCCTG5104.1152618E-415.7765435
CTAGTAC9005.2834366E-815.3257853
ACTGTCC7502.0594525E-615.3257858
ACTGTGT9758.4928615E-915.3257848
ACTCTGT7552.2068398E-615.224298
CCTATAC12104.0017767E-1115.1991273
TCTTAGA23500.015.1627452