FastQCFastQC Report
Mon 10 Oct 2016
332334Q_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename332334Q_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140291
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT852.73507E-454.4965442
TCTAGAA1152.0030762E-550.350072
TACTAAG1450.003784525231.9462492
GTGTAAG1903.8546693E-430.4750421
ACTATGG2750.005008648630.165915235
CCTTAGG2056.0142716E-428.2451612
TTAGACA1700.008234370527.2482724
GTGTAAC2607.6762735E-526.7242661
GTATATG2709.922324E-525.734481
TATGTAC2250.001035975125.734483
CCTACTA3751.3193785E-624.7051012
TACAGTG2650.002684875921.8500295
TACATTC2700.002992481221.44547
ATAGCAC3904.981314E-520.7855423
TACATAC2800.003694854720.6794912
TAGTAGA2800.003694854720.6794912
TTGTACT4557.067889E-620.3613454
CCTAAGG2850.004093724320.3166943
GTGCTTA4607.768313E-620.1400281
TACAGGG7002.1536835E-919.8523122