FastQCFastQC Report
Mon 10 Oct 2016
332334Q_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename332334Q_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140291
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTA852.7189087E-454.5619773
TAGACTT1150.001206274740.3284155
TATAGAT1701.992998E-434.1012342
TTTCATA2051.5032734E-533.9348872
GTATAGA1752.3628764E-433.126911
TCTATTA1400.003168224633.126912
TATACAT1853.2737266E-431.336272
GTGTAGA7350.029.9719681
TCTAGGG1550.005213957729.9210823
CTAGACA2353.8232516E-529.6027724
TAGACAG2803.789397E-628.9860485
ACTAGAC1650.007076496728.1076833
GTATATG2904.979327E-627.9865321
ATATAGA1700.008186310527.2809891
TATATGA2157.889388E-426.9637682
CCTAGAT1750.00942929126.501533
CCTAATG1750.00942929126.501531
TCTAGAT1750.00942929126.501532
GTCTTAT1750.00942929126.501531
TCTAGCT2209.0229657E-426.3509543