FastQCFastQC Report
Mon 10 Oct 2016
328777F_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename328777F_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699450
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA1051.6318882E-765.448141
TATTAAT1150.001281249839.8382
CATTAAA1300.00233956235.2413061
TATAAGA1300.00233956235.2413062
TTAATCA1802.9957737E-431.8150714
TATAGTC1500.004716000530.5424635
TCTAGGA3102.7567694E-729.5572242
GTGTTAA2209.6928806E-426.030511
TCCTAGA3109.1970705E-625.8625722
GTCTAGG5001.0932126E-925.1975351
TGTATGA6551.8189894E-1224.4806022
CCCTATA2400.001609565823.8613012
TAGTTTG2901.7375308E-423.6967417
TGTATAC4002.547229E-622.906852
TCTATAC3502.3543194E-522.906853
GTCTATA2550.002289956222.4576951
GTCCTAG4103.1563286E-622.3481461
AGTAATA2600.002563350622.0258162
GTCATAA3653.2555705E-521.9654731
GTATAGG4203.890191E-621.8160481