FastQCFastQC Report
Mon 10 Oct 2016
328777F_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename328777F_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699450
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAG500.002388455468.760433
GTATAAG1200.001574843138.2002371
TAAGAGT1806.6789544E-638.2002374
TGTTACA2206.315586E-736.4638632
TATACAT2206.315586E-736.4638632
TCCTATA1651.7912581E-434.727492
TACACTG1752.5307186E-432.7430575
ATACAGT3557.7852746E-1032.281896
TCCTAGA2501.71582E-632.08822
CTATGCT1500.004702635630.560194
TGTAAGA1500.004702635630.560192
TAATACC2703.127112E-629.7112964
GCTTAGT1600.00644670428.650181
TATTAGC1600.00644670428.650182
TCATAAG2005.534779E-428.6501772
GTATTAG2056.394767E-427.9513931
TAATGAG2056.394767E-427.9513932
TCTTAGG1750.00998179226.1944472
CCTTAGG2659.3846786E-525.9473321
GTACTAG2300.001251921924.9131981