FastQCFastQC Report
Mon 10 Oct 2016
327999Y_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename327999Y_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875856
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAG1100.001033886241.6141932
CTTAATA1501.0310719E-438.1463431
TATACTG1752.5518765E-432.6968655
ATAGTAG1954.8123088E-429.343343
TAAGGGT4553.6925485E-1027.6665764
GTACTAA1700.00872644426.926831
TAATGAA2659.475704E-525.910722
GTATACA4201.4329453E-724.522651
TATTACA2400.001617439723.8414652
GTCCTAG5302.1700544E-923.7514951
GTATTAC4902.4563633E-823.3549041
TTACAGT2600.00257585822.0075044
GTATAGA3153.0586068E-421.797911
TCCTAGA3703.6394573E-521.6506272
GTACATA3754.036387E-521.3619521
TAAGCCT3905.460344E-520.5403394
TATTGAG7251.7462298E-1020.5201022
CTAGTAC3354.635005E-420.496543
GTGTATA2850.00438586520.0770231
ATATAGA4209.652063E-519.0731721