FastQCFastQC Report
Mon 10 Oct 2016
327999Y_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename327999Y_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875856
Sequences flagged as poor quality0
Sequence length32-241
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAATA600.004949953457.2078132
TATTAGG1304.440338E-544.0060122
TATAGGA2601.3478711E-939.605412
CTACTAT1250.001939831936.6131
GTATAGG4008.185452E-1131.4642961
CGTTAAG2700.00422571531.234308235
AAGACTA1853.539479E-430.9231415
ATAGACT1853.539479E-430.9231414
GTATATG2703.1669715E-629.6633111
AATATTA1550.005564321329.5266152
GTAATAA1550.005564321329.5266151
TAAGACT1550.005564321329.5266154
TAGACTG1954.8180745E-429.337345
TTAGGAC2005.5875874E-428.6039053
ACTTACA2056.455742E-427.9062522
TCTAGGA2056.455742E-427.9062522
TAATAGA2158.527302E-426.6082842
GTATAAC2158.527302E-426.6082841
TTATACA2250.001111819325.4256954
ATATAGA4051.0099575E-725.4256941