FastQCFastQC Report
Mon 10 Oct 2016
316292K_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename316292K_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742584
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAATA956.3806165E-661.0746422
TATAGGA852.7070136E-454.607912
CTCTTAG650.006423779853.5577621
CATCTAA700.00861064649.7322041
CTAGACT1006.035708E-446.4167254
TATTGTG1006.035708E-446.4167255
TAAGGGT1150.001201022340.362374
GTGTAAT1601.3881584E-436.263071
GTCTAGA1300.002193379435.7051772
ATAAGAC1350.002639436634.382763
CCTAGAC1701.9825046E-434.1299443
CTATATC1701.9825046E-434.1299444
GTGTTAT1450.003746050632.0115361
GTATAGG1853.256525E-431.3626541
CCTCTAT1550.00519139429.9462761
GTCCATA2353.7995924E-529.6276971
ATAGGAC2404.3864064E-529.0104543
ATATAGC1650.007045924728.1313481
TAAGAGC1650.007045924728.1313483
TCCTGTA2505.793576E-527.8500372