FastQCFastQC Report
Mon 10 Oct 2016
316292K_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename316292K_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742584
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAAG150.00580561155.027135
TTTAGCT700.00854395449.830153
GCTATAT1109.558187E-442.280131
CCTCTAG1450.00371013832.0745771
GTCTAGA1550.005141697430.0052491
ACAGAGT1600.006004779629.0675898
TCAAATA1600.006004779629.0675892
CTCATAT1650.00697856928.1867521
GTCCTAG1700.00807310727.357731
TCTATGT2556.541819E-527.357732
ACTATGG1750.00929899926.5760782
TACTGTG1750.00929899926.5760782
TAGACAT2500.001869053623.254075
TCCATAT2550.002097146822.798112
GTAGACA4602.9537296E-722.7485474
TTCTGAG2600.002347654422.3596842
TGTAGGG3203.0535643E-421.8006922
GTAGGGA2700.002922734721.5315483
TTGAATG2700.002922734721.5315482
ATATCAG3253.3908908E-421.4652961